bs-4609R
[Primary Antibody]
CD105 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD105
Immunogen Range: 58-110/625
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2022
Swiss Prot: P17813
Source: KLH conjugated synthetic peptide derived from human CD105
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 70
Cross Reactive Species:
Human
Mouse
Rat
Dog
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Gao, Xin, et al. "Identification of Rat Respiratory Mucosa Stem Cells and Comparison of the Early Neural Differentiation Potential with the Bone Marrow Mesenchymal Stem Cells In Vitro." Cellular and Molecular Neurobiology: 1-12.Read more>>
- Wang, Kai, et al. "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells< i> in vitro</i>." Brain Research Bulletin (2013).Read more>>
- Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013).Read more>>
- Gao, Qian, et al. "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." Molecular biology reports 39.5 (2012): 5791-5804.Read more>>
- Gierloff, M., et al. "In vitro comparison of different carrier materials with rat bone marrow MSCs." Clinical Oral Investigations (2013): 1-13.Read more>>
- Zhao, Min, et al. "Placental expression of VEGF is increased in pregnancies with hydatidiform mole: Possible association with developing very early onset preeclampsia." Early Human Development (2013).Read more>>
- Bidkhori, Hamid Reza, et al. "Chemically primed bone-marrow derived mesenchymal stem cells show enhanced expression of chemokine receptors contributed to their migration capability." Iranian Journal of Basic Medical Sciences 19.1 (2016): 14-19.Read more>>
- Gao, Xin, et al. "Identification of rat respiratory Mucosa stem cells and comparison of the early neural differentiation potential with the bone marrow mesenchymal stem cells in vitro." Cellular and molecular neurobiology 34.2 (2014): 257-268Read more>>
- Wang, Zheng, et al. "Microparticle levels after arterial injury and NO therapy in diabetes." Journal of Surgical Research 200.2 (2016): 722-731.Read more>>
- Heirani-Tabasi, Asieh, et al. "Chemokine Receptors Expression in MSCs: Comparative Analysis in Different Sources and Passages." c14.5 (2017): 605-615.Read more>>
- Bach et al. Biologic canine and human intervertebral disc repair by notochordal cell-derived matrix: from bench towards bedside. (2018) Oncotarget. 9:26507-26526Read more>>
- Shao F et al. Multiboosting of cancer immunotherapy by a core-shell delivery system. Mol Pharm. 2020 Jan 6;17(1):338-348.Read more>>
- Ise H et al. Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin. Stem Cells International, 2019, 1–13.Read more>>
- Gae Won Parket al. Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns. Sci Rep. 2020 Jul 24;10(1):12448.Read more>>
- Keiko M et al. Cultured Neonatal Rat Cardiomyocytes Continue Beating Through Upregulation of CTGF Gene ExpressionJ Nippon Med Sch.2020;87(5):268-276.Read more>>
- Ruijing Zhao. et al. Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury. J Control Release. 2022 May;345:214Read more>>
- Park, Hyeri. et al. Increased phosphatase regenerating liver-1 trigger vascular remodeling in injured ovary via platelet-derived growth factor signaling pathway. Stem Cell Res Ther. 2022 Dec;13(1):1-16Read more>>
- Enoch Odame Anto. et al. Placental lesions and differential expression of pro-and anti-angiogenic growth mediators and oxidative DNA damage marker in placentae of Ghanaian suboptimal and optimal health status pregnant women who later developed preeclampsia. Plos One. 2022 Mar;17(3):e0265717Read more>>
- Jinpu Wei. et al. Biological characteristics of two mesenchymal stem cell cultures isolated from the umbilical cord and adipose tissue of a neonatal common hippo (Hippopotamus amphibius). TISSUE CELL. 2022 Aug;77:101862Read more>>
- Ziya Yurtal. et al. Protective and Therapeutic Effects of Bovine Amniotic Fluids Collected in Different Trimesters on The Epidural Fibrosis After Experimental Laminectomy in Rats. WORLD NEUROSURG. 2023 JaRead more>>
- Ma Caiyun. et al. Characteristics and Multilineage Differentiation of a Novel Type of Pulmonary Mesenchymal Stem Cells Derived from Goose Embryos. BRAZ ARCH BIOL TECHN. 2024 Apr;67:e24230139Read more>>
VALIDATION IMAGES
L1 rat brain, L2 rat heart lysates probed (bs-4609R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 70kDa.
Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-CD105 Polyclonal Antibody (bs-4609R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Image provided by One World Lab validation program. U138 cells probed with Rabbit Rabbit Anti-CD105 Polyclonal Antibody (bs-4609R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\n
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:Hela. <BR> Primary Antibody (green line): Rabbit Anti-CD105 antibody (bs-4609R) <BR> Dilution: 2ug/Test; <BR> Secondary Antibody : Goat anti-rabbit IgG-FITC<BR> Dilution: 0.5ug/Test. <br> Protocol<BR> The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.