bs-46018P

DATASHEET

Source: Recombinant SARS-COV-2 Spike RBD (N501Y, K417N, E484K) Protein protein is expressed in HEK293 with His tag. It contains the amino acid sequence of Arg319-Phe541 (N501Y, K417N, E484K).

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background:

SARS-CoV-2 exploits angiotensin-converting enzyme 2 (ACE2) as a receptor to invade cells. It has been reported that the UK and South African strains may have higher transmission capabilities, eventually in part due to amino acid substitutions on the SARS-CoV-2 Spike protein. The results of a study show the N501Y replacement in this region of the interface (present in both the UK and South African strains) should be favorable for the interaction with ACE2, while the K417N and E484K substitutions (South African strain) would seem neutral or even unfavorable.

Endotoxin: Less than 1EU per ug by the LAL method.

Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Sequence: Arg319-Phe541 (N501Y, K417N, E484K)

Expression: HEK293

Tag: His tag

Size: 1mg,100ug

Applications: WB()
ELISA()
HPLC()

Mol. Wt.: 26.2

Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.