bs-46011P [Protein]
Recombinant SARS-COV-2 S RBD (N501Y) Protein
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Entrez Gene: QHD43416.1

Source: Recombinant SARS-COV-2 S RBD (N501Y) Protein protein is expressed in HEK293 with His-Avi. It contains the amino acid sequence of Arg319 - Phe541 (N501Y)..

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background:

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Endotoxin: Less than 1EU per ug by the LAL method.

Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Sequence: Arg319 - Phe541 (N501Y).

Expression: HEK293

Tag: His-Avi

Size: 1mg,100ug

Applications: WB()
ELISA()
HPLC()

Mol. Wt.: 26.2


Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

SARS-COV-2 S protein RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.




The purity of SARS-COV-2 S protein RBD (N501Y) is greater than 95% as determined by SEC-HPLC.




Immobilized SARS-CoV-2 S RBD (N501Y) protein, His Tag at 0.5ug/ml (100ul/Well). Dose-response curve for ACE2, Fc tag with the EC50 of 12.7ng/ml determined by ELISA.