bs-46010P [Protein]
Recombinant SARS-COV-2 Spike S1 (D614G) Protein
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Swiss Prot: QHD43416.1

Source: Recombinant SARS-COV-2 Spike S1 (D614G) Protein protein is expressed in HEK293 with His-Avi. It contains the amino acid sequence of Gln14-Arg683.

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background:

The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

Endotoxin: Less than 1EU per ug by the LAL method.

Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Sequence: Gln14-Arg683

Expression: HEK293

Tag: His-Avi

Size: 1mg,100ug

Applications: WB()
ELISA()
HPLC()

Mol. Wt.: 77.9


Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Recombinant SARS-CoV-2 Spike S1 (D614G) Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.




The purity of Recombinant SARS-CoV-2 Spike S1 (D614G) Protein is greater than 95% as determined by SEC-HPLC.




Immobilized SARS-CoV-2 Spike S1 (D614G) at 2ug/ml (100ul/Well). Dose-response curve for Human ACE2, Fc tag with the EC50 of 42ng/ml determined by ELISA.