bs-4599R
[Primary Antibody]
MMP-2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MMP-2
Immunogen Range: 401-500/662
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4313
Swiss Prot: P08253
Source: KLH conjugated synthetic peptide derived from human MMP-2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 62
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Cavdar, Z., et al. "The effects of alpha-lipoic acid on MMP-2 and MMP-9 activities in a rat renal ischemia and re-perfusion model." Biotechnic & Histochemistry 0 (2013): 1-11.Read more>>
- Fan, Gaochao, et al. "Ultrasensitive photoelectrochemical immunoassay for matrix metalloproteinase-2 detection based on CdS: Mn/CdTe co-sensitized TiO2 nanotubes and signal amplification of SiO2@ Ab2 conjugates." Analytical Chemistry (2014).Read more>>
- Liu, Jianfeng, et al. "RECK is involved in intimal hyperplasia in carotid arteries: A new insight in the prevention of restenosis after vascular angioplasty."Annals of Vascular Surgery (2015).Read more>>
- Wang, Jing, et al. "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." BMC biology 15.1 (2017): 10.Read more>>
- Wang et al. The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma. (2017) J.Cell.Sci. 130:1078-1093Read more>>
- Bi et al. Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice. (2017) Sci.Rep. 7:10001Read more>>
- Gasparski et al. Transient mechanical strain promotes the maturation of invadopodia and enhances cancer cell invasionin vitro. (2017) J.Cell.Sci. 130:1965-1978Read more>>
- Yang X et al.Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cells. (2018)Biochem. Cell Biol. Read more>>
- Liu B et al. Interleukin-8 promotes prostate cancer bone metastasis through upregulation of bone sialoprotein. Oncol Lett. 2019 May;17(5):4607-4613. Read more>>
- Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332.Read more>>
- Xue Y et al. miR\205\5p inhibits psoriasis\associated proliferation and angiogenesis: Wnt/\catenin and mitogen\activated protein kinase signaling pathway are involved. J Dermatol. 2020 Aug;47(8):882-892.Read more>>
- Jia Guo. et al. Circ_0000140 restrains the proliferation, metastasis and glycolysis metabolism of oral squamous cell carcinoma through upregulating CDC73 via sponging miR-182-5p. Cancer Cell Int. 2020 Dec;20(1):1-12Read more>>
- Yang Zhang. et al. Plumbagin Inhibits Proliferation, Migration, and Invasion of Retinal Pigment Epithelial Cells Induced by FGF-2. Tissue Cell. 2021 Oct;72:101547Read more>>
- oyu Chen. et al. Study of the effects of rabbit scleral fibroblasts on cellular biomechanical properties and MMP-2 expression using two modes of riboflavin/ultraviolet A wave collagen cross-linking. Exp Eye Res. 2021 Jul;:108695Read more>>
- Xing Chen. et al. Transplantation of decellularized and lyophilized amniotic membrane inhibits endometrial fibrosis by regulating connective tissue growth factor and tissue inhibitor of matrix metalloproteinase?2. Exp Ther Med. 2021 Sep;22(3):1-8Read more>>
- Wei, Tao. et al. Two distinct males absent on the first (MOF)-containing histone acetyltransferases are involved in the epithelialmesenchymal transition in different ways in human cells. CELL MOL LIFE SCI. 2022 May;79(5):1-18Read more>>
- Yue-hua Liu. et al. ANKRD49 promotes the invasion and metastasis of lung adenocarcinoma via a P38/ATF-2 signalling pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2022 Jun 3Read more>>
- Ashley A. Thurstin. et al. Tissue Inhibitor of Metalloproteinase 4 (Timp4) Deletion in Mice Impacts Maternal Cardiac Function during Pregnancy and Postpartum. AM J PHYSIOL-HEART C. 2022 Dec;(Articles in Press)Read more>>
- Wang Wenjun. et al. LncRNA NEAT1 antagonizes the inhibition of melanoma proliferation, migration, invasion and EMT by Polyphyllin B. N-S ARCH PHARMACOL. 2023 Apr;:1-12Read more>>
- Sun Jia. et al. ANKRD49 promotes the metastasis of NSCLC via activating JNK-ATF2/c-Jun-MMP-2/9 axis. BMC CANCER. 2023 Dec;23(1):1-18Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-MMP-2 Polyclonal Antibody, Unconjugated (bs-4599R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Anti-MMP-2 Polyclonal Antibody, Unconjugated (bs-4599R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Rabbit heart lysates probed with Anti-MMP-2 Polyclonal Antibody, Unconjugated (bs-4599R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT
Paraformaldehyde-fixed, paraffin embedded rat bladder tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP-2 Polyclonal Antibody, Unconjugated (bs-4599R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: SY5Y cell lysates; Lane 2: U2os lysates; Lane 3: U251 cell lysates; Lane 4: Siha cell lysates probed with MMP-2 Polyclonal Antibody, Unconjugated (bs-4599R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MMP2)polyclonal Antibody, Unconjugated (bs-4599R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.