bs-4592R-FITC [Conjugated Primary Antibody]
CDKN2A/p16INK4a Polyclonal Antibody, FITC Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: CDKN2A/p16INK4a

Specificity: Targets p16INK4a

Immunogen Range: 31-100/156


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1029

Swiss Prot: P42771

Source: KLH conjugated synthetic peptide derived from human CDKN2A

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 15


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Yi, et al. "Pokemon enhances proliferation, cell cycle progression and anti-apoptosis activity of colorectal cancer independently of p14ARF–MDM2–p53 pathway." Medical Oncology 31.12 (2014): 1-8.Read more>>
  • Xuan Zheng. et al. Ultraviolet B irradiation up\regulates MM1 and induces photoageing of the epidermis. 2021 Feb 23Read more>>
VALIDATION IMAGES

Hela cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CDKN2A/p16INK4a Polyclonal Antibody(bs-4592R-FITC)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).