bs-4527R [Primary Antibody]
CSFV Envelope glycoprotein E2 Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: CSFV Envelope glycoprotein E2

Specificity: The immunogen for this antibody is sourced strain Brescia.

Immunogen Range: 951-1050/3898


Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P21530

Source: KLH conjugated synthetic peptide derived from CSFV Envelope glycoprotein E2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it?s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins.Envelope glycoprotein E2 exists in the form of homodimer; this protein is cleaved from the polyprotein by a cellular signal peptidase.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Virus
(CSFV strain Brescia)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiaodong Zou. et al. Cholesterol Biosynthesis Modulates CSFV Replication. VIRUSES-BASEL. 2022 Jul;14(7):145Read more>>
VALIDATION IMAGES

Transfected HeLa cell lysates probed with Anti-CSFV Envelope glycoprotein E2 Polyclonal Antibody, Unconjugated (bs-4527R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.


Lane 1: Recombinant CSFV E2 protein (200ng) (bs-20116P). Probed with CSFV E2 polyclonal Antibody, unconjugated (bs-4527R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.