Host: Rabbit
Target Protein: LCN2
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3934
Swiss Prot: P80188
Source: Recombinant human NGAL protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
Predicted Molecular Weight: 20
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LCN2) Polyclonal Antibody, Unconjugated (bs-43518R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.