Host: Rabbit
Target Protein: PRL
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5617
Swiss Prot: P01236
Source: Recombinant human PRL protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB()
IHC-P(1:200-400)
Predicted Molecular Weight: 24
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRL) Polyclonal Antibody, Unconjugated (bs-43099R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Recombinant human PRL protein, N-His (HEK293) (bs-43099P) probed with PRL Polyclonal Antibody, Unconjugated (bs-43099R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.