bs-4300R [Primary Antibody]
hnRNP L Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: hnRNP L

Immunogen Range: 501-589/589


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3191

Swiss Prot: P14866

Source: KLH conjugated synthetic peptide derived from human hnRNP L

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 65


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

L1 rat lung lysates L2 human colon carcinoma lysates probed with Anti hnRNP L Polyclonal Antibody, Unconjugated (bs-4300R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 65kD. Observed band size: 65kD