bs-4285R [Primary Antibody]
TPX2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TPX2

Immunogen Range: 551-650/747


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 22974

Source: KLH conjugated synthetic peptide derived from human TPX2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

TPX2 is essential for spindle pole and mitotic spindle formulation, playing a role in the S and G2 phases of mitosis. It is also critical for the function of protein kinase Aurora A. TPX2 autophosphorylates Aurora A, targeting it to the spindle apparatus and allowing it to function in eukaryotic meiotic and mitotic cell cycles. TPX2 has also been found to be highly expressed in cancer cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 86


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with TPX2 Polyclonal Antibody (bs-4285R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.