bs-4247R [Primary Antibody]
CHRNB Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CHRNB

Specificity: 100% sequence similarity with CHRNB2 and CHRNB4

Immunogen Range: 131-230/502


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1141, 1143

Source: KLH conjugated synthetic peptide derived from human CHRNB2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 52


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CHRNB2 Polyclonal Antibody, Unconjugated (bs-4247R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Mouse Cerebral cortex lysates; Lane 4: Rat Cerebrum lysates probed with CHRNB2 Polyclonal Antibody, Unconjugated (bs-4247R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.