Host: Rabbit
Target Protein: IL-18
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 29197
Swiss Prot: P97636
Source: Recombinant rat IL-18 protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Size: 100ul
Concentration: 1mg/ml
Applications:
ELISA(1:5000-10000)
IHC-P(1:100-500)
Predicted Molecular Weight: 22
Cross Reactive Species: Rat
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-18) Polyclonal Antibody, Unconjugated (bs-42148R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.