bs-4198R [Primary Antibody]
ETBR/Endothelin B Receptor Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ETBR/Endothelin B Receptor

Immunogen Range: 378-442/442


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1910

Swiss Prot: P24530

Source: KLH conjugated synthetic peptide derived from human Endolin B Receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)

Predicted Molecular Weight: 50


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ji L et al. A high affinity nanobody against endothelin receptor type B: a new approach to the treatment of melanoma. Mol Biol Rep. 2020 Mar;47(3):2137-2147. Read more>>
  • Ravi Kumar. et al. Induction of reactive oxygen species by mechanical stretch drives endothelin production in neonatal pig renal epithelial cells. REDOX BIOL. 2022 Sep;55:102394Read more>>
  • Nataliia Hula. et al. The Long-Term Effects of Prenatal Hypoxia on Coronary Artery Function of the Male and Female Offspring. BIOMEDICINES. 2022 Dec;10(12):3019Read more>>
VALIDATION IMAGES

Lane 1: Rat heart lysates; Lane 2: Mouse heart lysates probed with ETBR/Endothelin B Receptor Polyclonal Antibody, unconjugated (bs-4198R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Embryo tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Human A431 cell lysates ; Lane 6: Mouse BV-2 cell lysates; Lane 7: Human SH-SY5Y cell lysates probed with Endothelin B Receptor Polyclonal Antibody, Unconjugated (bs-4198R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.