DATASHEET
Host:
Rabbit
Target Protein:
YES1
Immunogen Range:
441-541/541
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
7525
Swiss Prot:
P07947
Source:
KLH conjugated synthetic peptide derived from human YES1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis.
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-YES1 Polyclonal Antibody, Unconjugated (bs-4166R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded mouse stomach labeled with Rabbit Anti-YES1 Polyclonal Antibody, Unconjugated (bs-4166R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti YES1 Polyclonal Antibody, Unconjugated (bs-4166R) at 1:200 followed by conjugation to the secondary antibody and DAB staining