bs-4163R-Biotin [Conjugated Primary Antibody]
JNK1 + JNK2 + JNK3 (T183 + T183 + T221) Polyclonal Antibody, Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: JNK1 + JNK2 + JNK3 (T183 + T183 + T221)

Modification Site: T183 + T183 + T221

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5599

Swiss Prot: P45983

Source: KLH conjugated Synthesised phosphopeptide derived from human JNK1/JNK2/JNK3 around the phosphorylation site of T183/T183/T221

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437.Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Gerson G Contreras-Chavez. et al. Changes in Appetite Regulation-Related Signaling Pathways in the Brain of Mice Supplemented with Non-nutritive Sweeteners. 2020 Oct 31Read more>>
  • Yan Zhang. et al. Polysaccharide from Ganoderma lucidum ameliorates cognitive impairment by regulating the inflammation of the brainCliver axis in rats. 2021 May 18Read more>>
  • Zhang, Rongrong. et al. Compound traditional Chinese medicine dermatitis ointment ameliorates inflammatory responses and dysregulation of itch-related molecules in atopic dermatitis. Chin Med-Uk. 2022 Dec;17(1):1-19Read more>>
  • Yanjie Wang. et al. Bisdemethoxycurcumin attenuates OVA_induced food allergy by inhibiting the MAPK and NF__B signaling pathways. EXP THER MED. 2022 Jun;23(6):1-8Read more>>
  • Lu Zhang. et al. Southern rice black-streaked dwarf virus induces incomplete autophagy for persistence in gut epithelial cells of its vector insect. PLOS PATHOG. 2023 Jan;19(1):e1011134Read more>>
  • Yang Liu. et al. Isochlorogenic Acid C Restrains Erk/JNK/NF-B Signaling to Alleviate Inflammatory Response and Promote Cell Apoptosis. J FOOD BIOCHEM. 2023;2023:5547108Read more>>
  • Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Keyi Nong. et al. Effect of the Pseudopleuronectes americanus-derived Pleurocidin on DSS-induced Ulcerative colitis in mice and its preliminary molecular mechanisms. INT IMMUNOPHARMACOL. 2024 Mar;130:111757Read more>>
VALIDATION IMAGES