bs-4123R [Primary Antibody]
MEK3(Thr222) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MEK3 Thr222

Modification Site: Thr222

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5606

Source: KLH conjugated synthetic phosphopeptide derived from human MEK3 around the phosphorylation site of Thr222

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 39


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Sheep
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Muscle tissue lysates; Lane 2: Rat Muscle tissue lysates probed with phospho-MEK3 (Thr222) Polyclonal Antibody, Unconjugated (bs-4123R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.