bs-4091R [Primary Antibody]
ASAP1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ASAP1

Immunogen Range: 154-190/1129


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 50807

Swiss Prot: Q9ULH1

Source: KLH conjugated synthetic peptide derived from human ASAP1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 125


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

MDA-MB-231 Cell lysates probed with ASAP1 Polyclonal Antibody, unconjugated (bs-4091R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded Mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ASAP1 Polyclonal Antibody, Unconjugated (bs-4091R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human Jurkat cell lysates probed with ASAP1 Polyclonal Antibody, Unconjugated (bs-4091R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.