bs-4036R [Primary Antibody]
PDHA1(Ser293) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PDHA1 Ser293

Specificity: This phosphorylation site is homologous across the listed species. This antibody will detect PDHA2, the testis specific form, when phosphorylated at Ser293 in Human, Ser296 in Mouse, and the predicted site of Ser296 in Rat due to sequence homology.

Modification Site: Ser293

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5160

Swiss Prot: P08559

Source: KLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 40


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yan-Zhu Chen. et al. Acrolein exposure affects ovarian function by interfering with glycolysis and mitochondrial energy metabolism in mouse. ENVIRON POLLUT. 2024 Nov;361:124776Read more>>
VALIDATION IMAGES

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Liver tissue lysates; Lane 5: Rat Muscle tissue lysates probed with phospho-PDHA1 (Ser293) Polyclonal Antibody, Unconjugated (bs-4036R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.