bs-4019R [Primary Antibody]
Thromboxane synthase Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Thromboxane synthase

Immunogen Range: 451-533/533


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6916

Swiss Prot: P24557

Source: KLH conjugated synthetic peptide derived from human Thromboxane synthase

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Thromboxane Synthase is a useful marker for the detection of native thromboxane synthase in smears, isolated cells, human tissue sections, and for affinity purification of the enzyme. In combination with the markers 27E10, RM 3/1 and 25F9, anti Thromboxane Synthase enables a more precise characterization of inflammatory processes in injured tissues, or in vitro cell-cell interaction studies.Distribution of thromboxane synthase in human tissues: Thromboxane synthase is predominantly produced by macrophages or antigen presenting cells of the myelo-monocytic lineage as shown below. Endothelial cells of placenta and epithelial cells in tonsils and bronchi also express this enzyme.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 59


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Akiko Furue. et al. Inhibition of TP signaling promotes endometriosis growth and neovascularization. MOL MED REP. 2023 Oct;28(4):1-14Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti CYP5A1/Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Antigen: bs-4019P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-295G-HRP) at 1: 5000; \nTMB staining; \nRead the data in MicroplateReader by 450nm. \n


A549 cell lysates probed with Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: U937 cell lysates; Lane 2: Mouse spleen lysates probed with Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.