bs-4010R [Primary Antibody]
ATG9A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ATG9A

Immunogen Range: 301-400/839


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 79065

Swiss Prot: Q7Z3C6

Source: KLH conjugated synthetic peptide derived from human ATG9A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 94


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti ATG9A Polyclonal Antibody, Unconjugated (bs-4010R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 94kD. Observed band size:94kD.


Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-ATG9A Polyclonal Antibody, Unconjugated (bs-4010R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Formalin-fixed and paraffin embedded rat stomach labeled with Rabbit Anti-ATG9A Polyclonal Antibody, Unconjugated (bs-4010R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n