bs-4002R
[Primary Antibody]
AMPK alpha-1/2 (Thr183/Thr172) Antibody
DATASHEET
Host: Rabbit
Target Protein: AMPK alpha-1/2 Thr183/Thr172
Modification Site: Thr172/Thr183
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5562
Source: KLH conjugated synthetic phosphopeptide derived from mouse AMPK alpha 2 around the phosphorylation site of Thr172
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 64
Cross Reactive Species:
Human
Mouse
Rat
Pig
Sheep
Predicted Cross Reactive Species:
Dog
Cow
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016).Read more>>
- Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.Read more>>
- Pandey, Aseem, et al. "Global Reprogramming of Host Kinase Signaling in Response to Fungal Infection." Cell Host & Microbe 21.5 (2017): 637-649.Read more>>
- Gingery, Anne, et al. "PDGFR Signaling Mediates Hyperproliferation and Fibrotic Responses of Subsynovial Connective Tissue Cells in Idiopathic Carpal Tunnel Syndrome." Scientific Reports 7.1 (2017): 16192.Read more>>
- Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
- Saito et al. PDGFR Signaling Mediates Hyperproliferation and Fibrotic Responses of Subsynovial Connective Tissue Cells in Idiopathic Carpal Tunnel Syndrome. (2017) Sci.Rep. 7:16192Read more>>
- Shi C et al. Acetaminophen aggravates fat accumulation in NAFLD by inhibiting autophagy via the AMPK/mTOR pathway. European Journal of Pharmacology.2019.Read more>>
- Viglino C et al. Chronic AICAR treatment prevents metabolic changes in cardiomyocytes exposed to free fatty acids. Pflugers Arch. 2019 May 31. Read more>>
- Qi H et al. MSTN attenuates cardiac hypertrophy through inhibition of excessive cardiac autophagy by blocking AMPK/mTOR and miR-128/PPARγ/NF-κB signaling pathways. Mol Ther Nucleic Acids. 2019 Dec 14;19:507-522. Read more>>
- Tang S et al. High ammonia exposure regulates lipid metabolism in the pig skeletal muscle via mTOR pathway. Sci Total Environ. 2020 Oct 20;740:139917.Read more>>
- Prado-Garcia H et al. Lactic Acidosis in the Presence of Glucose Diminishes Warburg Effect in Lung Adenocarcinoma Cells. Front Oncol. 2020 Jun 12;10:807.Read more>>
- Li T et al. Fufang-Zhenzhu-Tiaozhi capsule ameliorates rabbits iliac artery restenosis by regulating adiponectin signaling pathway. Biomed Pharmacother. 2020 Aug;128:110311.Read more>>
- El-Ghaiesh SE et al. Metformin Protects From RotenoneCInduced Nigrostriatal Neuronal Death in Adult Mice by Activating AMPK-FOXO3 Signaling and Mitigation of Angiogenesis. Front Mol Neurosci. 2020 Jun 18;13:84.Read more>>
- Weiyi Zhanget al. Cinnamaldehyde changes the dynamic balance of glucose metabolism by targeting ENO1. Life Sci. 2020 Oct 1;258:118151.Read more>>
- Liu J et al. Obesity Potentiates Esophageal Squamous Cell Carcinoma Growth and Invasion by AMPK-YAP PathwayJ Immunol Res.2020 Dec 10;2020:6765474.Read more>>
- abak Baban. et al. AMPK induces regulatory innate lymphoid cells after traumatic brain injury. Jci Insight. 2021 Jan;6(1):126766Read more>>
- Jiao Jiao Zhang. et al. Estradiol ameliorates metformin-inhibited Sertoli cell proliferation via AMPK/TSC2/mTOR signaling pathway. Theriogenology. 2021 Nov;175:7Read more>>
- Songsong Jiang. et al. A Comparison Study on the Therapeutic Effect of High Protein Diets Based on Pork Protein versus Soybean Protein on Obese Mice. FOODS. 2022 Jan;11(9):1227Read more>>
- Jian Chen. et al. Astragalus polysaccharide alleviates transport stress-induced heart injury in newly hatched chicks via ERS-UPR-Autophagy dependent pathway. POULTRY SCIENCE. 2022 Jun;:10203Read more>>
- Jianfei Pan. et al. Fecal Microbiota Was Reshaped in UCP1 Knock-In Pigs via the Adipose-Liver-Gut Axis and Contributed to Less Fat Deposition. MICROBIOL SPECTR. 2023 Jan 23Read more>>
- Khan, Mohammad Badruzzaman. et al. Exercise Improves Cerebral Blood Flow and Functional Outcomes in an Experimental Mouse Model of Vascular Cognitive Impairment and Dementia (VCID). TRANSL STROKE RES. 2023 Jan;:1-16Read more>>
- Yani He. et al. Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-B signaling pathway. INT IMMUNOPHARMACOL. 2023 Jun;119:110192Read more>>
- Shuaiqi Han. et al. Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells. ECOTOX ENVIRON SAFE. 2023 Jul;259:115063Read more>>
- Weipeng Zhang. et al. Cross-generational effects of dietary sea buckthorn on non-alcoholic fatty liver disease in offspring of obese female mice. J FUNCT FOODS. 2024 Oct;121:106398Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Antibody (bs-4002R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
HepG2 cell lysates probed with phospho-AMPK alpha-2 (Thr172) Polyclonal Antibody, Unconjugated (bs-4002R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebral cortex lysates probed with AMPK alpha-1/2 (Thr183/Thr172) Polyclonal Antibody, Unconjugated (bs-4002R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Polyclonal Antibody, Unconjugated (bs-4002R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Polyclonal Antibody, Unconjugated (bs-4002R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Polyclonal Antibody, Unconjugated (bs-4002R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Polyclonal Antibody, Unconjugated (bs-4002R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Eye lysates; Lane 2: Rat Muscle lysates; Lane 3: Rat Heart lysates; Lane 4: Rat Kidney lysates; Lane 5: Rat Eye lysates probed with AMPK alpha-1/2 Polyclonal Antibody, Unconjugated (bs-4002R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AMPK alpha-1/2 (Thr183/Thr172) Antibody(bs-4002R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).