bs-4001R [Primary Antibody]
HNF4 (Ser313) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HNF4 Ser313

Modification Site: Ser313

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3172

Source: KLH conjugated synthetic phosphopeptide derived from human HNF4 alpha around the phosphorylation site of Ser313

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 52


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Pig
Horse
Chicken
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang C et al. Caveolin-1 promotes Rfng expression via Erk-Jnk-p38 signaling pathway in mouse hepatocarcinoma cells. J Physiol Biochem. 2019 Sep 16.Read more>>
  • Qidang Duan. et al. LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4 signaling in HLSECs. HELIYON. 2022 Dec;8:e12385Read more>>
VALIDATION IMAGES

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Rat Stomach tissue lysates probed with phospho-HNF4 (Ser313) Polyclonal Antibody, Unconjugated (bs-4001R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.