Host: Rabbit
Target Protein: Glucagon Receptor
Immunogen Range: 11-110/477
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2642
Swiss Prot: P47871
Source: KLH conjugated synthetic peptide derived from human Glucagon Receptor
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 51
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Chicken
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Rafacho, Alex, et al. "Pancreatic Alpha-Cell Dysfunction Contributes to the Disruption of Glucose Homeostasis and Compensatory Insulin Hypersecretion in Glucocorticoid-Treated Rats." PLOS ONE 9.4 (2014): e93531.Read more>>
- Tao Wu. et al. Lactobacillus rhamnosus LRa05 Ameliorate Hyperglycemia through a Regulating Glucagon-Mediated Signaling Pathway and Gut Microbiota in Type 2 Diabetic Mice. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
Formalin-fixed and paraffin embedded rat heart labeled with Anti Glucagon Receptor Polyclonal Antibody, Unconjugated (bs-3945R) followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with Glucagon Receptor Polyclonal Antibody, unconjugated (bs-3945R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Glucagon Receptor Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Blank control:HepG2. Primary Antibody (green line): Rabbit Anti-Glucagon Receptor antibody (bs-3945R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Protocol Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Glucagon Receptor Polyclonal Antibody(bs-3945R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).