Host: Rabbit
Target Protein: Legumain
Immunogen Range: 201-300/433
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5641
Swiss Prot: Q99538
Source: KLH conjugated synthetic peptide derived from human Legumain
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
Predicted Molecular Weight: 46
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Horse
For research use only. Not intended for diagnostic or therapeutic use.
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Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti Legumain Polyclonal Antibody, Unconjugated (bs-3907R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 49kD. Observed band size: 49kD.
Moues kidney lysates probed with Legumain Polyclonal Antibody, unconjugated (bs-3907R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse placenta lysates probed with Legumain Polyclonal Antibody, unconjugated (bs-3907R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti Legumain Polyclonal Antibody, Unconjugated (bs-3907R) followed by conjugation to the secondary antibody and DAB staining
HL-60 cells were fixed with 4% PFA for 10min at room temperature, 0.2% PBST for 20 min at room temperature. 5% BSA blocking buffer was used for 30 min at room temperature. Cells were then stained with Legumain Polyclonal Antibody (bs-3907R) at 1:33 dilution for 30 min at room temperature and then washed twice with 2% BSA in PBS. Secondary Antibody (PE-conjugated) incubation was performed for 40 min at room temperature. Acquisitions of 20,000 events were performed. The graph shows cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells(black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Legumain Polyclonal Antibody, A488 Conjugated (bs-3907R-A488) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Legumain) Polyclonal Antibody, Unconjugated (bs-3907R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human JAR cell lysates; Lane 2: Human MCF-7 cell lysates probed with Legumain Polyclonal Antibody, Unconjugated (bs-3907R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.