bs-39011R [Primary Antibody]
phospho-BHMT1(Thr45) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: phospho-BHMT1(Thr45)

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 635

Swiss Prot: Q93088

Source: KLH conjugated Synthesised phosphopeptide derived from human BHMT1 around the phosphorylation site of Thr45

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)

Predicted Molecular Weight: 45


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.