bs-3900R [Primary Antibody]
CYP2R1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CYP2R1

Immunogen Range: 251-350/501


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 120227

Source: KLH conjugated synthetic peptide derived from human CYP2R1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are mono-oxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor.Defects in CYP2R1 are a cause of 25-hydroxyvitamin D(3) deficiency, also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. First described in patients who had rickets at a young age despite a history of adequate vitamin D intake. The patients sera had low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activity and low levels of 25-hydroxyvitamin D.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Stenhouse Claire. et al. Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling. Biol Reprod. 2022 FebRead more>>
  • Wenjing Lu. et al. Seasonal changes of vitamin D3 and ovarian steroidogenesis in the wild ground squirrels (Citellus dauricus Brandt). J STEROID BIOCHEM. 2023 Nov;234:106385Read more>>
  • Wenjing Lu. et al. Vitamin D status alters genes involved in ovarian steroidogenesis in muskrat granulosa cells. BBA-MOL CELL BIOL L. 2024 May;1869:159469Read more>>
VALIDATION IMAGES

Lane 1: A431 cell lysates probed with CYP2R1 Polyclonal Antibody, Unconjugated (bs-3900R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: A431 cell lysates probed with CYP2R1 Polyclonal Antibody, Unconjugated (bs-3900R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.