bs-3884R-Cy5
[Conjugated Primary Antibody]
GPX4 Polyclonal Antibody, Cy5 Conjugated
DATASHEET
Host: Rabbit
Target Protein: GPX4
Immunogen Range: 101-197/197
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2879
Source: KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.
Conjugation: Cy5
Excitation/ Emission: 625,650nm/670nm
Size: 100ul
Concentration: 1ug/ul
Applications: WB(1:300-5000)
Predicted Molecular Weight: 22
Cross Reactive Species: Mouse
Predicted Cross Reactive Species:
Human
Rat
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Sukfan P. Kwong. et al. PORIMIN: The key to (+)-Usnic acid-induced liver toxicity and oncotic cell death in normal human L02 liver cells. J Ethnopharmacol. 2021 Apr;270:113873Read more>>
- Jing Ge. et al. Comparative study on protective effect of different selenium sources against cadmium-induced nephrotoxicity via regulating the transcriptions of selenoproteome. Ecotox Environ Safe. 2021 Jun;215:112135Read more>>
- Andrea Krehe?ov. et al. Characterization of Glutathione Peroxidase 4 in Rat Oocytes, Preimplantation Embryos, and Selected Maternal Tissues during Early Development and Implantation. Int J Mol Sci. 2021 Jan;22(10):5174Read more>>
- Xiao Han. et al. ZEB1 directly inhibits GPX4 transcription contributing to ROS accumulation in breast cancer cells. Breast Cancer Res Tr. 2021 Jul;188(2):329-342Read more>>
- Tao Liu. et al. Multifaceted roles of a bioengineered nanoreactor in repressing radiation-induced lung injury. Biomaterials. 2021 Oct;277:121103Read more>>
- Lijuan Bao. et al. Ferritinophagy is involved in Bisphenol A-induced ferroptosis of renal tubular epithelial cells through the activation of the AMPK-mTOR-ULK1 pathway. Food Chem Toxicol. 2022 May;163:112909Read more>>
- Jianwei Lei. et al. The protective mechanism of protein kinase R to inhibit neuronal ferroptosis in cerebral injury from subarachnoid hemorrhage. BRAIN BEHAV. 2022 Jul;:e2722Read more>>
- Huang Bin. et al. Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization. OXID MED CELL LONGEV. 2022;2022:9735555Read more>>
- Zhaoqi He. et al. Cadmium induces liver dysfunction and ferroptosis through the endoplasmic stress-ferritinophagy axis. ECOTOX ENVIRON SAFE. 2022 Oct;245:114123Read more>>
- Zhang Kaixin. et al. MiR-129-3p regulates ferroptosis in the liver of Selenium-deficient broilers by targeting SLC7A11. POULTRY SCIENCE. 2022 Oct;:102271Read more>>
- Hu, Xiaomeng. et al. PNO1 inhibits autophagy-mediated ferroptosis by GSH metabolic reprogramming in hepatocellular carcinoma. CELL DEATH DIS. 2022 Nov;13(11):1-12Read more>>
- Yajing Li. et al. Effect of Zearalenone-Induced Ferroptosis on Mice Spermatogenesis. ANIMALS. 2022 Jan;12(21):3026Read more>>
- Shuang Wang. et al. Dioscin Protects against Cisplatin-Induced Acute Kidney Injury by Reducing Ferroptosis and Apoptosis through Activating Nrf2/HO-1 Signaling. ANTIOXIDANTS-BASEL. 2022 Dec;11(12):2443Read more>>
- Ke Li. et al. Oxygen Self-Generating Nanoreactor Mediated Ferroptosis Activation and Immunotherapy in Triple-Negative Breast Cancer. ACS NANO. 2023;17(5):4667C4687Read more>>
- Xu Chen. et al. Chiral Ruthenium Nanozymes with Self-Cascade Reaction Driven the NO Generation Induced Macrophage M1 Polarization Realizing the Lung Cancer Cocktail Therapy. SMALL. 2023 Apr;:2207823Read more>>
- Lu Yu. et al. Chronic arsenic exposure induces ferroptosis via enhancing ferritinophagy in chicken livers. SCI TOTAL ENVIRON. 2023 May;:164172Read more>>
- Li Zhang. et al. Tumor-Associated Macrophages Regulating a Polymer Nanoplatform for Synergistic Treatment of Breast Tumors. ACS APPL MATER INTER. 2023;15(29):34527C34539Read more>>
- Yu-Sheng Shi. et al. Dendrobine rescues cognitive dysfunction in diabetic encephalopathy by inhibiting ferroptosis via activating Nrf2/GPX4 axis. PHYTOMEDICINE. 2023 Jul;:154993Read more>>
- Zhong Gaolong. et al. Copper Exposure Induced Chicken Hepatotoxicity: Involvement of Ferroptosis Mediated by Lipid Peroxidation, Ferritinophagy, and Inhibition of FSP1-CoQ10 and Nrf2/SLC7A11/GPX4 Axis. BIOL TRACE ELEM RES. 2023 Jul;:1-11Read more>>
- Mali Qiu. et al. YAP facilitates NEDD4L-mediated ubiquitination and degradation of ACSL4 to alleviate ferroptosis in myocardial ischemia-reperfusion injury. CAN J CARDIOL. 2023 AugRead more>>
- Yang Liu. et al. Tuberostemonine may enhance the function of the SLC7A11/glutamate antiporter to restrain the ferroptosis to alleviate pulmonary fibrosis. J ETHNOPHARMACOL. 2023 Aug;:116983Read more>>
- Yueqi Yang. et al. A Compared Study of Eicosapentaenoic Acid and Docosahexaenoic Acid in Improving Seizure-Induced Cognitive Deficiency in a Pentylenetetrazol-Kindling Young Mice Model. MAR DRUGS. 2023 Sep;21(9):464Read more>>
- Lihua Zhao. et al. Saikosaponin A alleviates Staphylococcus aureus-induced mastitis in mice by inhibiting ferroptosis via SIRT1/Nrf2 pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 AugRead more>>
- Ting Hong. et al. Exosomal circBBS2 inhibits ferroptosis by targeting miR-494 to activate SLC7A11 signaling in ischemic stroke. FASEB J. 2023 Aug;37(9):e23152Read more>>
- Huan Yang. et al. Heat stress induces ferroptosis of porcine Sertoli cells by enhancing CYP2C9-Ras- JNK axis. THERIOGENOLOGY. 2024 Feb;215:281Read more>>
- Yang Liu. et al. Inhibition of Oxidative Stress-Induced Ferroptosis Can Alleviate Rheumatoid Arthritis in Human. J IMMUNOL RES. 2024;2024:9943747Read more>>
- Lan Jing. et al. Neuropeptide substance P attenuates colitis by suppressing inflammation and ferroptosis via the cGAS-STING signaling pathway. INT J BIOL SCI. 2024 Apr;20(7):2507-2531Read more>>
- Lu Zhang. et al. The EGR1/miR-139/NRF2 axis orchestrates radiosensitivity of non-small-cell lung cancer via ferroptosis. CANCER LETT. 2024 May;:217Read more>>
- Qian-zhuo Liu. et al. Berberine alleviates ovarian tissue damage in mice with hepatolenticular degeneration by suppressing ferroptosis and endoplasmic reticulum stress. J INTEGR MED-JIM. 2024 MayRead more>>
- Zhen Chen. et al. AQP4 regulates ferroptosis and oxidative stress of Muller cells in diabetic retinopathy by regulating TRPV4. EXP CELL RES. 2024 May;:114087Read more>>
- Cuicui Zhuang. et al. Escherichia coli infection induces ferroptosis in bovine mammary epithelial cells by activating the Wnt/-catenin pathway-mediated mitophagy. MITOCHONDRION. 2024 Sep;78:101921Read more>>
- Lijuan Bao. et al. Ferroptosis is involved in Staphylococcus aureus-induced mastitis through autophagy activation by endoplasmic reticulum stress. INT IMMUNOPHARMACOL. 2024 Oct;140:112818Read more>>
- Xunhu Gu. et al. METTL14-Mediated m6A Modification of TUG1 Represses Ferroptosis in Alzheimer's Disease via Inhibiting GDF15 Ubiquitination. FRONT BIOSCI-LANDMRK. 2024 Aug;29(8):298Read more>>
VALIDATION IMAGES