Host: Rabbit
Target Protein: Cytochrome b245 Light Chain
Immunogen Range: 21-120/195
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1535
Swiss Prot: P13498
Source: KLH conjugated synthetic peptide derived from human CK18 Cytochrome b245 Light Chain/p22phox
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 21
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Walton, Noah M., et al. "Adult neurogenesis transiently generates oxidative stress." PloS one 7.4 (2012): e35264.Read more>>
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- Yue, Long, et al. "Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways." International Journal of Biological Macromolecules (2016).Read more>>
- Yoshida, Toshinori, et al. "Apocynin and enzymatically modified isoquercitrin suppress the expression of a NADPH oxidase subunit p22phox in steatosis-related preneoplastic liver foci of rats." Experimental and Toxicologic Pathology (2016).Read more>>
- Song Q et al. Potential mechanisms underlying the protective effects of salvianic acid A against atherosclerosis in vivo and vitro. (2019)Biomedicine & Pharmacotherapy, 109, 945–956. Read more>>
- Xiaoyu Liu. et al. Geniposide Combined With Notoginsenoside R1 Attenuates Inflammation and Apoptosis in Atherosclerosis via the AMPK/mTOR/Nrf2 Signaling Pathway. Front Pharmacol. 2021; 12: 687394Read more>>
- Xin Guo. et al. Aspirin protects human trophoblast HTR-8/SVneo cells from H2O2-Induced oxidative stress via NADPH/ROS pathway. PLACENTA. 2023 Dec;144:55Read more>>
This image was generously provided by Ao Li, MD from Chongqing University of Technology. Serum-starved HK-2 cells were pretreated with DMSO (vehicle) or ATG (2 μM) for 4 h, and then incubated with 10 ng/ml TGF-β1 for 0.25–24 hr
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Cytochrome b245 Light Chain Polyclonal Antibody, Unconjugated (bs-3879R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.