Host: Rabbit
Target Protein: SH2D2A
Immunogen Range: 101-200/389
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9047
Source: KLH conjugated synthetic peptide derived from human SH2D2A
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 43
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Compeer et al. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. (2018) Nat.Commun. 9:1597Read more>>
- Compeer et al. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. (2018) Nat.Commun. 9:1597Read more>>
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SH2D2A Polyclonal Antibody(bs-3858R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).