DATASHEET
Host:
Rabbit
Target Protein:
HNF4A
Immunogen Range:
301-400/464
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
3172
Swiss Prot:
P41235
Source:
KLH conjugated synthetic peptide derived from human HNF4 alpha
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
PRODUCT SPECIFIC PUBLICATIONS
- Zhang C et al. Caveolin-1 promotes Rfng expression via Erk-Jnk-p38 signaling pathway in mouse hepatocarcinoma cells. J Physiol Biochem. 2019 Sep 16.Read more>>
- Johanna Vollmar. et al. Functional inhibition of Oct leads to HNF4 upregulation. Exp Ther Med. 2021 Apr;21(4):1-1Read more>>
- Lv, Xiaoqin. et al. GATA binding protein 4 promotes the expression and transcription of hepatitis B virus by facilitating hepatocyte nuclear factor 4 alpha in vitro. Virol J. 2021 Dec;18(1):1-12Read more>>
- Qidang Duan. et al. LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4 signaling in HLSECs. HELIYON. 2022 Dec;8:e12385Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human kidney labeled with Anti-HNF4/TCF4/HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
BRL-3A cell lysates probed with HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hcclm3 cell lysates probed with HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.