Host: Rabbit
Target Protein: Oct-4BOCT4B-190NT
Immunogen Range: 201-300/360
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5460
Swiss Prot: Q01860
Source: KLH conjugated synthetic peptide derived from human OCT4B-190
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 21/39
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Liu, Yu-Hong, et al. "A signature for induced pluripotent stem cell?Cassociated genes in colorectal cancer."Medical Oncology 30.1 (2013): 1-11.eRead more>>
- Darabi, Shahram, et al. "Polarized neural stem cells derived from adult bone marrow stromal cells develop a rosette-like structure." In Vitro Cellular & Developmental Biology-Animal (2013): 1-15.Read more>>
- Tanabe, A., et al. "Radioresistance of cancer stem‐like cell derived from canine tumours." Veterinary and Comparative Oncology (2014).Read more>>
- Liu, Lu, et al. "OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation." BioMed Research International 2017 (2017).Read more>>
- Liu et al. JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells. (2015) Acta.Pharmacol.Si. 36:1099-106Read more>>
- Liu et al. OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation. (2017) Biomed.Res.Int. 2017:2756891Read more>>
- Xiuxiu Liu. et al. PHF20 inhibition promotes apoptosis and cisplatin chemosensitivity via the OCT4?p?STAT3?MCL1 signaling pathway in hypopharyngeal squamous cell carcinoma. Int J Oncol. 2021 Jul;59(1):1-14Read more>>
- Jiejuan Lai. et al. Comparison of the biological and functional characteristics of mesenchymal stem cells from intrahepatic and identical bone marrow. Stem Cell Res. 2021 Aug;55:102477Read more>>
Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti OCT4B-190 Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti OCT4B-190 Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC96 probed with Oct-4B(OCT4B-190)Polyclonal Antibody, Unconjugated (bs-3816R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Cerebellum lysates; Lane 4: Mouse Testis lysates; Lane 5: Rat Cerebrum lysates; Lane 6: Rat Heart lysates; Lane 7: Rat Cerebellum lysates probed with Oct-4B(OCT4B-190)Polyclonal Antibody, Unconjugated (bs-3816R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Oct-4B(OCT4B-190) Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Oct-4B(OCT4B-190) Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Oct-4B(OCT4B-190) Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Oct-4B(OCT4B-190) Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 overnight at 4°C, DAB staining.