bs-3806R
[Primary Antibody]
UCHL1/PGP9.5 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: UCHL1/PGP9.5
Immunogen Range: 35-140/223
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7345
Swiss Prot: P09936
Source: KLH conjugated synthetic peptide derived from human PGP9.5
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 25
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Chicken
Goat
Cow
Predicted Cross Reactive Species:
Pig
Horse
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zheng, Liming, et al. "CD49f promotes proliferation of male dairy goat germline stem cells." Cell Proliferation (2016).Read more>>
- Li, Bo, et al. "Bovine male germline stem-like cells cultured in serum-and feeder-free medium." Cytotechnology: 1-13.Read more>>
- Deng, Shou‐Long, et al. "Melatonin promotes development of haploid germ cells from early developing spermatogenic cells of Suffolk sheep under in vitro condition." Journal of Pineal Research (2016).Read more>>
- Zheng, Liming, et al. "The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation." Scientific Reports 6 (2016): 37414.Read more>>
- Lei, Qi-jing, et al. "Establishment and characterization of immortalized bovine male germline stem cell line." Journal of Integrative Agriculture (2017).Read more>>
- Yang F et al. Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis. Brazilian Journal of Poultry Science.2019 Feb.Read more>>
- Ko-Chao Lee. et al. Use of iTRAQ-based quantitative proteomic identification of CHGA and UCHL1 correlated with lymph node metastasis in colorectal carcinoma. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 MayRead more>>
- Zixiu Liu. et al. NGF Signaling Exacerbates KOA Peripheral Hyperalgesia via the Increased TRPV1-Labeled Synovial Sensory Innervation in KOA Rats. PAIN RES MANAG. 2024;2024:1552594Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-UCHL1/PGP9.5 Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (UCHL1/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (UCHL1/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (UCHL1/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (UCHL1/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (UCHL1/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (UCHL1/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (UCHL1/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.