bs-3776R [Primary Antibody]
Histone H3 (acetyl K9) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Histone H3 acetyl K9

Specificity: This acetylation site is homologous across the species listed.

Modification Site: acetyl K9

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3020

Swiss Prot: P84243

Source: KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys9

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 15


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Rabbit
Drosophila

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yeo, Abrey J., et al. "Senataxin controls meiotic silencing through ATR activation and chromatin remodeling." Cell Discovery 1 (2015).Read more>>
  • Li R et al. Effects of PM2.5 exposure in utero on heart injury, histone acetylation and GATA4 expression in offspring mice. Chemosphere.2020 Oct;256:127133.Read more>>
  • Jinbing Xue. et al. A novel histone deacetylase inhibitor LT-548-133-1 induces apoptosis by inhibiting HDAC and interfering with microtubule assembly in MCF-7 cells. 2021 Mar 31Read more>>
  • Xia Liu. et al. Luteolin alleviates non-alcoholic fatty liver disease in rats via restoration of intestinal mucosal barrier damage and microbiota imbalance involving in gut-liver axis. Arch Biochem Biophys. 2021 Oct;711:109019Read more>>
VALIDATION IMAGES

Lane 1:Huh7 lysates Lane 2: A549 lysates probed with Rabbit Anti-Histone H3 (acetyl K9) Polyclonal Antibody, Unconjugated (bs-3776R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Lane 1: NIH/3T3 cell lysates; Lane 2:Hela cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: U2os cell lysates probed with Histone H3 (acetyl K9) Polyclonal Antibody, Unconjugated (bs-3776R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.