Host: Rabbit
Target Protein: PPAR Gamma ser112
Modification Site: ser112
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5468
Swiss Prot: P37231
Source: KLH conjugated synthetic phosphopeptide derived from human PPAR Gamma around the phosphorylation site of ser112
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 57
Cross Reactive Species:
Human
Mouse
Rat
Dog
Predicted Cross Reactive Species:
Pig
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Mölzer, Christine, et al. "Features of an altered AMPK metabolic pathway in Gilbert’s Syndrome, and its role in metabolic health." Scientific Reports 6 (2016): 30051.Read more>>
- Manabe A et al.Upregulation of transient receptor potential melastatin 6 channel expression by rosiglitazone and all-trans-retinoic acid in erlotinib-treated renal tubular epithelial cells.(2018)J Cell Physiol. Read more>>
- Liu Y et al. Telmisartan inhibits oxalate and calcium oxalate crystal-induced epithelial-mesenchymal transformation via PPAR-γ-AKT/STAT3/p38 MAPK-Snail pathway. Life Sci. 2019 Nov 28;241:117108.Read more>>
- Min Zhao et al. HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway. Mediators of Inflammation, 2019, 1–17. Read more>>
- Liu YD et al. Rosiglitazone Suppresses Calcium Oxalate Crystal Binding and Oxalate-Induced Oxidative Stress in Renal Epithelial Cells by Promoting PPAR-γ Activation and Subsequent Regulation of TGF-β1 and HGF Expression. Oxid Med Cell Longev. 2019 Nov 12;2019:4826525.Read more>>
- Hsu MH et al. Effects of Maternal Resveratrol on Maternal High-Fat Diet/Obesity with or without Postnatal High-Fat Diet. Int J Mol Sci. 2020 May 12;21(10):3428.Read more>>
- Yurong Zhao. et al. Deoxynivalenol Exposure Suppresses Adipogenesis by Inhibiting the Expression of Peroxisome Proliferator-Activated Receptor Gamma 2 (PPAR2) in 3T3-L1 Cells. Int J Mol Sci. 2020 Jan;21(17):63Read more>>
- Fadian Ding. et al. Adropin attenuates pancreatitis?associated lung injury through PPAR phosphorylation?related macrophage polarization. INT J MOL MED. 2023 Oct;52(4):1-1Read more>>
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Phospho-PPAR Gamma(ser112) Polyclonal Antibody, Unconjugated (bs-3737R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Phospho-PPAR Gamma (ser112) Polyclonal Antibody, Unconjugated (bs-3737R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-3737P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450
Lane 1: Human A549 cell lysates; Lane 2: Mouse Large intestine lysates; Lane 3: Rat Large intestine lysates; Lane 4: Rat Lung lysates probed with PPAR Gamma Polyclonal Antibody, Unconjugated (bs-3737R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-PPAR Gamma (Ser112)) polyclonal Antibody, Unconjugated (bs-3737R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.