bs-3731R-Cy3 [Conjugated Primary Antibody]
eNOS (Thr495) Polyclonal Antibody, Cy3 Conjugated
www.biossusa.com
[email protected]
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+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: eNOS Thr495

Modification Site: Thr495

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4846

Swiss Prot: P29474

Source: KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Thr495

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 133


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Papinska, A. M., et al. "Angiotensin‐(1‐7) administration benefits cardiac, renal, and progenitor cell function in db/db mice." British Journal of Pharmacology(2015).Read more>>
  • Jie Chen. et al. Triterpenoid saponins from Ilex pubescens promote blood circulation in blood stasis syndrome by regulating sphingolipid metabolism and the PI3K/AKT/eNOS signaling pathway. PHYTOMEDICINE. 2022 Jun;:154242Read more>>
VALIDATION IMAGES