Host: Rabbit
Target Protein: BRN3A
Immunogen Range: 325-419/419
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5457
Swiss Prot: Q01851
Source: KLH conjugated synthetic peptide derived from human BRN3A
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 43
Cross Reactive Species:
Human
Mouse
Rat
Pig
Predicted Cross Reactive Species:
Dog
Cow
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Xue, Yuanchao, et al. "Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells." Nature Neuroscience (2016).Read more>>
- Bell, Katharina, et al. "Neuroprotective effects of antibodies on retinal ganglion cells in an adolescent retina organ culture." Journal of Neurochemistry (2016).Read more>>
- Bell et al. Neuroprotective effects of antibodies on retinal ganglion cells in an adolescent retina organ culture. (2016) J.Neurochem. 139:256-269Read more>>
- Sujia Ma. et al. Effect of papaverine on axonal outgrowth of primary retinal ganglion cells of Sprague Dawley rats. Exp Eye Res. 2021 Oct;:108797Read more>>
- Siqi Li. et al. Structural damage to the rat eye following long-term simulated weightlessness. EXP EYE RES. 2022 Oct;223:1092Read more>>
- Zhuhe Liu. et al. CXCL7 aggravates the pathological manifestations of neuromyelitis optica spectrum disorder by enhancing the inflammatory infiltration of neutrophils, macrophages and microglia. CLIN IMMUNOL. 2022 Sep;:109139Read more>>
- Yifan Song. et al. Matrine promotes mitochondrial biosynthesis and reduces oxidative stress in experimental optic neuritis. FRONT PHARMACOL. 2022; 13: 936632Read more>>
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti BRN3A Polyclonal Antibody, Unconjugated (bs-3669R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 43kD. Observed band size: 43kD.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-3669R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-3669R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(bs-3669R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).