bs-3648R-BF405
[Conjugated Primary Antibody]
KEAP1 Polyclonal Antibody, AbBy Fluor® 405 Conjugated
DATASHEET
Host: Rabbit
Target Protein: KEAP1
Immunogen Range: 65-160/624
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9817
Swiss Prot: Q14145
Source: KLH conjugated synthetic peptide derived from human KEAP1
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Conjugation: AbBy Fluor® 405
Excitation/ Emission: 401nm/421nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 69
Cross Reactive Species:
Human
Mouse
Rat
Pig
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Su, Chuanyang, et al. "Tetrachlorobenzoquinone activates Nrf2 signaling by Keap1 cross-linking and ubiquitin translocation, but not Keap1-Cullin3 complex dissociation." Chemical Research in Toxicology (2015).Read more>>
- Su, Chuanyang, et al. "Neohesperidin dihydrochalcone against CCl4-induced hepatic injury through different mechanisms The implication of free radical scavenging and Nrf2 activation." Journal of Agricultural and Food Chemistry(2015).Read more>>
- Chen, Ya-Hui, et al. "Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia." PLOS ONE11.4 (2016): e0154210.Read more>>
- Su, Chuanyang, et al. "The electrophilic character of quinones is essential for the suppression of Bach1." Toxicology (2017).Read more>>
- Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653Read more>>
- Meng et al. Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1. (2018) J.Anim.Sci.Biotechnol. 9:34Read more>>
- Kacar S et al. Effect of acrylamide on BEAS-2B normal human lung cells: Cytotoxic, oxidative, apoptotic and morphometric analysis. Acta Histochem. 2019 Jul;121(5):595-603. Read more>>
- Zhao Y et al. Lycopene prevents DEHP-induced Leydig cell damage with the Nrf2 antioxidant signaling pathway in mice. J Agric Food Chem. 2019 Dec 8.Read more>>
- Chen W et al. Obeticholic Acid Protects against Gestational Cholestasis-Induced Fetal Intrauterine Growth Restriction in Mice. Oxidative Medicine and Cellular Longevity, 2019, 1–17. Read more>>
- Jin Houet al. Silver Nanoparticles Induced Oxidative Stress and Mitochondrial Injuries Mediated Autophagy in HC11 Cells Through Akt/AMPK/mTOR Pathway. Biol Trace Elem Res. 2021 Mar;199(3):1062-1073.Read more>>
- Junrong Zhang. et al. Neuroprotective effects of maize tetrapeptide-anchored gold nanoparticles in Alzheimers disease. Colloid Surface B. 2021 Apr;200:111584Read more>>
- Jacopo Di Gregorio. et al. UBXN7 cofactor of CRL3KEAP1 and CRL2VHL ubiquitin ligase complexes mediates reciprocal regulation of NRF2 and HIF-1 proteins. Bba-Mol Cell Res. 2021 Apr;1868:118963Read more>>
- Yingran Liang. et al. Crocin ameliorates arsenic trioxide?induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis. Biomed Pharmacother. 2020 Nov;131:110713Read more>>
- Kaiqiang Fu. et al. Tanshinone IIA exhibits anti-inflammatory and antioxidative effects in LPS-stimulated bovine endometrial epithelial cells by activating the Nrf2 signaling pathway. Res Vet Sci. 2021 May;136:22Read more>>
- Feng Gao. et al. Aucubin ameliorates the LPS\induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF\B and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27Read more>>
- Song Yang. et al. Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-B signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo. J Ethnopharmacol. 2021 Oct;:114739Read more>>
- Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-B signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965Read more>>
- Fen Xu. et al. Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway. Exp Cell Res. 2022 Mar;412:113025Read more>>
- Xinyue Chang. et al. The Protective Effect of Trichilia catigua A. Juss. on DEHP-Induced Reproductive System Damage in Male Mice. Front Pharmacol. 2022; 13: 832789Read more>>
- Huiling Mao. et al. Leucine protects bovine intestinal epithelial cells from hydrogen peroxide-induced apoptosis by alleviating oxidative damage. J SCI FOOD AGR. 2022 May 05Read more>>
- Yiyun Qian. et al. Seeds of Ginkgo biloba L. inhibit oxidative stress and inflammation induced by cigarette smoke in COPD rats through the Nrf2 pathway. J ETHNOPHARMACOL. 2023 Jan;301:115758Read more>>
- Shikuo Rong. et al. Amentoflavone Exerts Anti-Neuroinflammatory Effects by Inhibiting TLR4/MyD88/NF-<i></i>B and Activating Nrf2/HO-1 Pathway in Lipopolysaccharide-Induced BV2 Microglia. MEDIAT INFLAMM. 2022 Dec 06;2022:5184721Read more>>
- Huang, Jianjia. et al. Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways. J ZHEJIANG UNIV-SC B. 2023 Feb;24(2):157-171Read more>>
- Xiaoxiao Zou. et al. Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice. ANTIOXIDANTS-BASEL. 2023 Mar;12(3):712Read more>>
- Chen Chunni. et al. Study on the targeted regulation of Scutellaria baicalensis leaf on glutamineCglutamate metabolism and glutathione synthesis in the liver of D-gal ageing rats. J PHARM PHARMACOL. 2023 JuRead more>>
- Haichao Wang. et al. The gut-liver axis perspective: Exploring the protective potential of polysaccharides from Cistanche deserticola against alcoholic liver disease. INT J BIOL MACROMOL. 2024 Jan;256:128394Read more>>
- Hao Hao. et al. Protective effects of chlorogenic acid against cyclophosphamide induced liver injury in mice. BIOTECH HISTOCHEM. 2023 Nov 29Read more>>
- Jie Lv. et al. Yunvjian decoction mitigates hyperglycemia in rats induced by a high-fat diet and streptozotocin via reducing oxidative stress in pancreatic beta cells. J ETHNOPHARMACOL. 2024 Jun;327:118045Read more>>
- Chong Zhao. et al. MetalOrganic Framework-Capped Gold Nanorod Hybrids for Combinatorial Cancer Therapy. MOLECULES. 2024 Jan;29(10):2384Read more>>
- Zhiyuan Xiao. et al. BMSC-derived exosome overexpressing GATA-4 suppresses H/R-induced cardiomyocyte ferroptosis through miR-330-3p/BAP1/SLC7A11/IP3R axis and mPTP opening. ISCIENCE. 2024 22Read more>>
VALIDATION IMAGES