bs-3614R
[Primary Antibody]
PPAR alpha Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PPAR alpha
Immunogen Range: 301-400/468
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5465
Swiss Prot: Q07869
Source: KLH conjugated synthetic peptide derived from human PPAR alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 51
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Others
Predicted Cross Reactive Species:
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Renu K et al. Elevated lipolysis in adipose tissue by doxorubicin via PPARα activation associated with hepatic steatosis and insulin resistance.(2019) Eur J Pharmacol;843:162-176.Read more>>
- Yan R et al. PM2. 5 exposure induces age-dependent hepatic lipid metabolism disorder in female mice. journal of environmental science.2019.Read more>>
- Lv N et al. Perfluorooctanoic acid-induced toxicities in chicken embryo primary cardiomyocytes: Roles of PPAR alpha and Wnt5a/Frizzled2. Toxicol Appl Pharmacol. 2019 Aug 21;381:114716.Read more>>
- "Wang L et al. Antiobesity, Regulation of Lipid Metabolism, and Attenuation of Liver Oxidative Stress Effects of Hydroxy-α-sanshool Isolated from Zanthoxylum bungeanum on High-Fat Diet-Induced Hyperlipidemic Rats. Oxidative Medicine and Cellular Longevity. 2019 ID 5852494."Read more>>
- Li T et al. Fufang-Zhenzhu-Tiaozhi capsule ameliorates rabbits iliac artery restenosis by regulating adiponectin signaling pathway. Biomed Pharmacother. 2020 Aug;128:110311.Read more>>
- Tan W et al. Pyrazinamide alleviates rifampin-induced steatohepatitis in mice by regulating the activities of cholesterol-activated 7-hydroxylase and lipoprotein lipase. Eur J Pharm Sci. 2020 Aug 1;151:105402.Read more>>
- Shogo Kumagaiet al. An Oncogenic Alteration Creates a Microenvironment that Promotes Tumor Progression by Conferring a Metabolic Advantage to Regulatory T Cells. Immunity. 2020 Jul 14;53(1):187-203.e8.Read more>>
- Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci. 2020 Oct 1;258:118147.Read more>>
- Liu H et al. Cybrid Model Supports Mitochondrial Genetic Effect on Pig Litter SizeFront Genet.2020 Dec 15;11:579382.Read more>>
- Qionghua Hu. et al. Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis. J Surg Res. 2021 Sep;265:223Read more>>
- Cao Zhanhong. et al. Protective Effects of Huangqi Shengmai Yin on Type 1 Diabetes-Induced Cardiomyopathy by Improving Myocardial Lipid Metabolism. Evid-Based Compl Alt. 2021;2021:5590623Read more>>
- Hu, Shuang. et al. Deletion of p38 attenuates ethanol consumption- and acetaminophen-induced liver injury in mice through promoting Dlg1. Acta Pharmacol Sin. 2021 Nov;:1-16Read more>>
- Zhou, Jielin. et al. The daily caloric restriction and alternate-day fasting ameliorated lipid dysregulation in type 2 diabetic mice by downregulating hepatic pescadillo 1. Eur J Nutr. 2022 Mar;:1-23Read more>>
- Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292Read more>>
- Shu-Yan Gao. et al. iTRAQ Quantitative Proteomic Analysis of Different Expressed Proteins and Signal Pathways in Bakuchiol-Induced Hepatotoxicity. EVID-BASED COMPL ALT. 2022 Sep 19;2022:292824Read more>>
- Lili Chang. et al. Regulating T-cell metabolic reprogramming and blocking PD-1 co-promote personalized postoperative autologous nanovaccines. BIOMATERIALS. 2023 Jun;297:122104Read more>>
- Jie Luo. et al. Copper nanoparticles lead to reproductive dysfunction by affecting key enzymes of ovarian hormone synthesis and metabolism in female rats. ECOTOX ENVIRON SAFE. 2023 Apr;254:114704Read more>>
- Qian Chen. et al. Changes to PUFA-PPAR pathway during mesaconitine induced myocardial coagulative necrosis. FOOD CHEM TOXICOL. 2023 May;:113831Read more>>
- Xiaotong Su. et al. DMC (2,4-dihydroxy-6-methoxy-3,5-dimethylchalcone) enhances exercise tolerance via the AMPK-SIRT1-PGC-1 pathway in mice fed a high-fat diet. PHYTOTHER RES. 2023 JuRead more>>
- Chunqiu Fang. et al. Tiaogan Jiejiu Tongluo Formula attenuated alcohol-induced chronic liver injury by regulating lipid metabolism in rats. J ETHNOPHARMACOL. 2023 Dec;317:116838Read more>>
- Qiao Jingyi. et al. Mulberry fruit repairs alcoholic liver injury by modulating lipid metabolism and the expression of miR-155 and PPAR in rats. FUNCT INTEGR GENOMIC. 2023 Sep;23(3):1-11Read more>>
- Xiaobo Zhang. et al. Integrated network pharmacology, metabolomics, and transcriptomics of Huanglian-Hongqu herb pair in non-alcoholic fatty liver disease. J ETHNOPHARMACOL. 2024 May;325:117828Read more>>
VALIDATION IMAGES
Lane 1: Mouse heart lysates Lane 2: Mouse liver lysates probed with Rabbit Anti-PPAR alpha Polyclonal Antibody, Unconjugated (bs-3614R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Arctic Ground Squirrel splenocytes stained with Anti-PPAR alpha Polyclonal Antibody, PE-CY5 Conjugated (bs-3614R-PE-Cy5) at 1:50.
Rat splenocytes stained with Anti-PPAR alpha Polyclonal Antibody, PE-CY5 Conjugated (bs-3614R-PE-Cy5) at 1:50.
Mouse heart lysates probed with Rabbit Anti-PPAR alpha Polyclonal Antibody, Unconjugated (bs-3614R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.
A431 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PPAR alpha Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-3614R-AF647) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates; Lane 3: Mouse Liver lysates; Lane 4: Mouse Urinary bladder lysates ; Lane 5: Mouse Kidney lysates probed with PPAR alpha Polyclonal Antibody, Unconjugated (bs-3614R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PPAR alpha Polyclonal Antibody(bs-3614R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).