bs-3608R [Primary Antibody]
CYP11A1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CYP11A1

Immunogen Range: 321-420/521


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1583

Swiss Prot: P05108

Source: KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 53/57


Cross Reactive Species: Human
Mouse
Rat
Bovine

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hu, Yanhui, et al. "Low-dose monobutyl phthalate stimulates steroidogenesis through steroidogenic acute regulatory protein regulated by SF-1, GATA-4 and C/EBP-beta in mouse Leydig tumor cells." Reproductive Biology and Endocrinology 11.1 (2013): 72.Read more>>
  • Mahata, Bidesh, et al. "Single-Cell RNA Sequencing Reveals T Helper Cells Synthesizing Steroids De Novo to Contribute to Immune Homeostasis." Cell Reports (2014).Read more>>
  • Medwid, Samantha, Haiyan Guan, and Kaiping Yang. "Prenatal exposure to bisphenol A disrupts adrenal steroidogenesis in adult mouse offspring."Environmental Toxicology and Pharmacology (2016).Read more>>
  • Ommati, Mohammad Mehdi, et al. "Dual effects of sulfasalazine on rat sperm characteristics, spermatogenesis, and steroidogenesis in two experimental models." Toxicology letters (2017).Read more>>
  • Sagaradze GD et al. Application of rat cryptorchidism model for the evaluation of mesenchymal stromal cell secretome regenerative potential.(2018)Biomed Pharmacother;109:1428-1436. Read more>>
  • "Jeremy M et al. Vitamin D3 mediated regulation of steroidogenesis mitigates testicular activity in an aged rat model.J Steroid Biochem Mol Biol. 2019 Jun;190:64-75."Read more>>
  • Sun J et al. Icariin protects mouse Leydig cell testosterone synthesis from the adverse effects of di (2-ethylhexyl) phthalate. Toxicol Appl Pharmacol. 2019 Jun 6;378:114612. Read more>>
  • Huang CC et al. Hyperactive CREB signaling pathway involved in the pathogenesis of polycystic ovarian syndrome revealed by patient-specific induced pluripotent stem cell modeling. Fertil Steril. 2019 Jul 2. pii: S0015-0282(19)30428-5. Read more>>
  • Wang X et al. Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells. Int J Biol Sci. 2019 Jan 1;15(2):404-415.Read more>>
  • Xiaopeng An. et al. Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1. J Anim Sci Biotechno. 2020 Dec;11(1):1-2Read more>>
  • Jianyu Ma. et al. Melatonin alleviated oxidative stress induced by energy restriction on sheep Leydig cells through Sirt1/Sod2 pathway. Theriogenology. 2021 JulRead more>>
  • Zhou, Xiaofeng. et al. DNMT1-mediated lncRNA IFFD controls the follicular development via targeting GLI1 by sponging miR-370. CELL DEATH DIFFER. 2022 Dec;:1-13Read more>>
  • Sailing Chen. et al. Biocompatible Assessment of Erythrocyte Membrane-Camouflaged Polymeric PLGA Nanoparticles in Pregnant Mice: Both on Maternal and Fetal/Juvenile Mice. INT J NANOMED. 2022 Dec;17:5899-5913Read more>>
  • Chuckles Ch. Marak. et al. Effect of Cycas pectinata Seed Extract on Testicular Steroidogenesis in a Mouse Model. ANDROLOGIA. 2023;2023:5446928Read more>>
  • Kehan Wang. et al. Autophagy regulation and protein kinase activity of PIK3C3 controls sertoli cell polarity through its negative regulation on SCIN (scinderin). AUTOPHAGY. 2023 Jul 14Read more>>
  • Yan-Zhu Chen. et al. Acrolein exposure affects ovarian function by interfering with glycolysis and mitochondrial energy metabolism in mouse. ENVIRON POLLUT. 2024 Nov;361:124776Read more>>
VALIDATION IMAGES

Mouse placenta lysates probed with Rabbit Anti-CYP11A1 Polyclonal Antibody, Unconjugated (bs-3608R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.


Lane 1: mouse kidney lysates Lane 2: human kidney lysates probed with Anti CYP11A1/P450SCC Polyclonal Antibody, Unconjugated (bs-3608R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 53/57kD. Observed band size: 53kD.\n


Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CYP11A1 Polyclonal Antibody, Unconjugated (bs-3608R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (bs-3608R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining


Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (bs-3608R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining