bs-3605R
[Primary Antibody]
YAP1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: YAP1
Immunogen Range: 131-330/504
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 10413
Swiss Prot: P46937
Source: KLH conjugated synthetic peptide derived from human YAP1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Li, Hongxin, et al. "gga-miR-375 Plays a Key Role in Tumorigenesis Post Subgroup J Avian Leukosis Virus Infection." PLoS One 9.4 (2014): e90878.Read more>>
- Gao R et al. PM2.5-associated nitro-PAH exposure promotes tumor cell metastasis through Hippo-YAPmediated transcriptional regulation. Sci Total Environ. 2019 Aug 15;678:611-617. Read more>>
- Sisi Liuet al. Geometric Confinement Guides the Expression of Cancer Stem Cell Molecular Markers CD44 via Cell Traction Forces. ACS Biomater Sci Eng. 2020 Aug 10;6(8):4623-4630.Read more>>
- Liu J et al. Obesity Potentiates Esophageal Squamous Cell Carcinoma Growth and Invasion by AMPK-YAP PathwayJ Immunol Res.2020 Dec 10;2020:6765474.Read more>>
- Qi Yang. et al. A novel biodegradable external stent regulates vein graft remodeling via the Hippo-YAP and mTOR signaling pathways. Biomaterials. 2020 Nov;258:120254Read more>>
- Xinhao Wang. et al. Yes-associated protein reacts differently in vascular smooth muscle cells under different intensities of mechanical stretch. Aging-Us. 2022 Jan 15; 14(1): 286296Read more>>
- Zixin Liu. et al. Polyisocyanide hydrogels with tunable nonlinear elasticity mediate liver carcinoma cell functional response. ACTA BIOMATER. 2022 JuRead more>>
- Ruixing Liu. et al. Celastrol Alleviates Corneal Stromal Fibrosis by Inhibiting TGF-1/Smad2/3-YAP/TAZ Signaling After Descemet Stripping Endothelial Keratoplasty. INVEST OPHTH VIS SCI. 2023 Mar;64(3):9-9Read more>>
- Ximin Yuan. et al. Tough Gelatin Hydrogel for Tissue Engineering. Advanced Science. 2023 Jun;:2301665Read more>>
- Shao Zifei. et al. Exosomes derived from adipose tissues accelerate fibroblasts and keratinocytes proliferation and cutaneous wound healing via miR-92a/Hippo-YAP axis. J PHYSIOL BIOCHEM. 2023 Dec;:1-16Read more>>
- Shun Zhang. et al. Hnrnpk Protects Against Osteoarthritis through Targeting WWC1 mRNA and Inhibiting Hippo Signaling Pathway. MOL THER. 2024 Feb 26Read more>>
- Yiwei Wang. et al. M2 macrophages promote subconjunctival fibrosis through YAP/TAZ signalling. ANN MED. 2024 Feb 09Read more>>
- Xie Zheng. et al. Liquid crystal matrix-based viscoelastic mechanical stimulation regulates nuclear localization and osteogenic differentiation of rBMSCs. CELLULOSE. 2024 May;31(8):5229-5248Read more>>
- Yiwei Wang. et al. Celastrol alleviates subconjunctival fibrosis induced by silicone implants mimicking glaucoma surgery. EUR J PHARM BIOPHARM. 2024 Jun;:114352Read more>>
- Yanyu Chen. et al. A novel mechanism of ferroptosis inhibition-enhanced atherosclerotic plaque stability: YAP1 suppresses vascular smooth muscle cell ferroptosis through GLS1. FASEB J. 2024 Aug;38(15):e2385Read more>>
- Xie Binqing. et al. Cyclic tensile stress promotes osteogenic differentiation via upregulation of Piezo1 in human dental follicle stem cells. HUM CELL. 2024 Aug;:1-14Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti-YAP1 Polyclonal Antibody, Unconjugated(bs-3605R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-YAP1 Polyclonal Antibody, Unconjugated(bs-3605R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-YAP1 Polyclonal Antibody, Unconjugated (bs-3605R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (YAP1) polyclonal Antibody, Unconjugated (bs-3605R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.