bs-3602R-APC [Conjugated Primary Antibody]
ATG1/ULK1 Polyclonal Antibody, APC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ATG1/ULK1

Immunogen Range: 951-1050/1050


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8408

Swiss Prot: O75385

Source: KLH conjugated synthetic peptide derived from human ATG1/ULK1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Conjugation: APC

Excitation/ Emission: 650nm/660nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 116


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Song, Fuyong, et al. "Involvement of autophagy in tri-ortho-cresyl phosphate-induced delayed neuropathy in hens." Neurochemistry International (2013).Read more>>
  • Gao, Yuan, et al. "Activation of lysosomal degradative pathway in spinal cord tissues of carbon disulfide-treated rats." Chemico-Biological Interactions (2014).Read more>>
  • Shen, P. P., et al. "Cortical Spreading Depression (CSD) Preconditioning Mediates Neuroprotection against Ischemic Stroke by Inducing AMP‐activated Protein Kinase‐dependent Autophagy in a Rat Cerebral Ischemic/reperfusion Injury Model." Journal of Neurochemistry (2016).Read more>>
  • Shen et al. Cortical spreading depression preconditioning mediates neuroprotection against ischemic stroke by inducing AMP-activated protein kinase-dependent autophagy in a rat cerebral ischemic/reperfusion injury model. (2017) J.Neurochem. 140:799-813Read more>>
  • olin Cai. et al. LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with sarcoplasmic/endoplasmic reticulum calcium ATPase 2. Mol Ther-Nucl Acids. 2021 DecRead more>>
  • Cai, Bolin. et al. Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development. CELL DEATH DIS. 2022 Apr;13(4):1-12Read more>>
  • Jia Song. et al. Mesenchymal stromal cells ameliorate diabetes-induced muscle atrophy through exosomes by enhancing AMPK/ULK1-mediated autophagy. J CACHEXIA SARCOPENI. 2023 JaRead more>>
  • Ma Manting. et al. LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers. CELL COMMUN SIGNAL. 2023 Dec;21(1):1-16Read more>>
  • Chunli Yang. et al. BMAL1 involved in autophagy and injury of thoracic aortic endothelial cells of rats induced by intermittent heat stress through the AMPK/mTOR/ULK1 pathway. BIOCHEM BIOPH RES CO. 2023 Jun;661:34Read more>>
  • Ting Chen. et al. Xiaojianzhong decoction attenuates aspirin-induced gastric mucosal injury via the PI3K/AKT/mTOR/ULK1 and AMPK/ULK1 pathways. PHARM BIOL. 2023;61(1):1234-1248Read more>>
  • Huang Qun. et al. Bushenhuoluo Decoction improves polycystic ovary syndrome by regulating exosomal miR-30a-5p/ SOCS3/mTOR/NLRP3 signaling-mediated autophagy and pyroptosis. J OVARIAN RES. 2024 Dec;17(1):1-17Read more>>
  • Huan Deng. et al. Low selenium and T-2 toxin may be involved in the pathogenesis of Kashin-Beck disease by affecting AMPK/mTOR/ULK1 pathway mediated autophagy. ECOTOX ENVIRON SAFE. 2024 Jul;279:116503Read more>>
  • Lu Man. et al. Promotion and Mechanism of Acupotomy on Chondrocyte Autophagy in Knee Osteoarthritis Rabbits. CHIN J INTEGR MED. 2024 Jun;:1-9Read more>>
VALIDATION IMAGES