Host: Rabbit
Target Protein: eNOS Thr113
Specificity: This phosphorylation site is homologous across the listed species; this phosphorylation site does not exist in human due to a Thr-Ser substitution.
Modification Site: Thr113
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 18127
Swiss Prot: P70313
Source: KLH conjugated synthetic phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser113
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 133
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Yi Ren. et al. Pravastatin attenuates sepsis-induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of Cav-1/eNOS pathway. Int Immunopharmacol. 2021 Nov;100:108077Read more>>
- Zhongxiao Zhou. et al. Formononetin regulates endothelial nitric oxide synthase to protect vascular endothelium in deep vein thrombosis rats:. INT J IMMUNOPATH PH. 2022;()Read more>>
- Zhongxiao Zhou. et al. Formononetin regulates endothelial nitric oxide synthase to protect vascular endothelium in deep vein thrombosis rats:. INT J IMMUNOPATH PH. 2022;()Read more>>
- Yang Wang. et al. Role of GADD45A in myocardial ischemia/reperfusion through mediation of the JNK/p38 MAPK and STAT3/VEGF pathways. INT J MOL MED. 2022 Dec;50(6):1-11Read more>>
- Zhou Yanyan. et al. Homoplantaginin attenuates high glucose-induced vascular endothelial dysfunction via inhibiting store-operated calcium entry channel and endoplasmic reticulum stress. J PHARM PHARMACOL. 2023 SepRead more>>
- Lingchao Kong. et al. Tibial cortex transverse transport regulates Orai1/STIM1-mediated NO release and improve the migration and proliferation of vessels via increasing osteopontin expression. J ORTHOP TRANSL. 2024 Mar;45:107Read more>>
Lane 1: Rat brain lysates; Lane 2: Rat liver lysates probed with Rabbit Anti-eNOS (Thr113) Polyclonal Antibody (bs-3589R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with eNOS (Thr113) Polyclonal Antibody(bs-3589R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).