bs-3586R [Primary Antibody]
Tuberin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Tuberin

Immunogen Range: 1601-1750/1809


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7249

Swiss Prot: P49815

Source: KLH conjugated synthetic peptide derived from human Tuberin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 200


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Yuanyuan, et al. "Repression of TSC1/TSC2 mediated by MeCP2 regulates human embryo lung fibroblast cell differentiation and proliferation." International Journal of Biological Macromolecules (2016).Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled withAnti-Tuberin/TSC2 Polyclonal Antibody, Unconjugated(bs-3586R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human gastric adenocarcinoma tissue labeled with Anti-Tuberin/TSC2 Polyclonal Antibody, Unconjugated(bs-3586R) at 1:300, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human endometrial tissue labeled with Anti-Tuberin/TSC2 Polyclonal Antibody, Unconjugated(bs-3586R) at 1:300, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tuberin Polyclonal Antibody, Unconjugated (bs-3586R) at 1:200 overnight at 4°C, DAB staining.