bs-3550R-BF647 [Conjugated Primary Antibody]
Myogenin Polyclonal Antibody, AbBy Fluor® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Myogenin

Immunogen Range: 80-125/224


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4656

Swiss Prot: P15173

Source: KLH conjugated synthetic peptide derived from human MYOG/Myogenin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).

Conjugation: AbBy Fluor® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 25


Cross Reactive Species: Human
Mouse
Rat
Bovine

Predicted Cross Reactive Species: Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li S et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019 Apr 25.Read more>>
  • Li S et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019 Apr 25.Read more>>
  • Wang Y et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019 Nov 7;10(11):852.Read more>>
  • Weng S et al. Improvement of muscular atrophy by AAVCSaCas9-mediated myostatin gene editing in aged mice. Cancer Gene Ther. 2020 Dec;27(12):960-975.Read more>>
  • Zhao Wang. et al. Fibronectin type III domain-containing 4 promotes the migration and differentiation of bovine skeletal muscle-derived satellite cells via focal adhesion kinase. Cell Adhes Migr. 2020;14(1):153-164Read more>>
  • Jie Li. et al. MicroRNA-127-3p regulates myoblast proliferation by targeting Sept7. Biotechnol Lett. 2020 Sep;42(9):1633-1644Read more>>
  • Qiang Gao. et al. Myogenin suppresses apoptosis induced by angiotensin II in human induced pluripotent stem cell-derived cardiomyocytes. Biochem Bioph Res Co. 2021 May;552:84Read more>>
  • Genghua Chen. et al. Transcriptome profile analysis reveals KLHL30 as an essential regulator for myoblast differentiation. Biochem Bioph Res Co. 2021 Jun;559:84Read more>>
  • Kuan-Xiang Sun. et al. Deletion of phosphatidylserine flippase -subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration. Zool Res. 2021 Sep 18; 42(5): 650C659Read more>>
  • Zhi Hu. et al. MUSTN1 is an indispensable factor in the proliferation, differentiation and apoptosis of skeletal muscle satellite cells in chicken. Exp Cell Res. 2021 Oct;407:112833Read more>>
  • Yilei Li. et al. Vitellogenin 2 promotes muscle development and stimulates the browning of white fat. Aging-Us. 2021 Oct 15; 13(19): 22985C23003Read more>>
  • Jing Zhang. et al. LncRNA SMARCD3-OT1 Promotes Muscle Hypertrophy and Fast-Twitch Fiber Transformation via Enhancing SMARCD3X4 Expression. INT J MOL SCI. 2022 Jan;23(9):451Read more>>
  • Sheng-Zhong Han. et al. miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation. J AGR FOOD CHEM. 2022;70(33):1012110133Read more>>
  • Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858Read more>>
  • Pengkang Song. et al. Vitamin A injection at birth improves muscle growth in lambs. ANIM NUTR. 2023 JuRead more>>
  • Genghua Chen. et al. Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation. CELL PROLIFERAT. 2023 Sep;:e13545Read more>>
  • Yue Wu. et al. The biological characteristics of chicken embryo mesenchymal stem cells isolated from chorioallantoic membrane. GENESIS. 2024 Apr;62(2):e23592Read more>>
  • Changbin Zhao. et al. IGF2 promotes the differentiation of chicken embryonic myoblast by regulating mitochondrial remodeling. J CELL PHYSIOL. 2024 JuRead more>>
  • Junfang Zhang. et al. GSK3 regulation Wnt/-catenin signaling affects adipogenesis in bovine skeletal muscle fibro/adipogenic progenitors. INT J BIOL MACROMOL. 2024 Aug;275:133639Read more>>
  • Lixia Sun. et al. Delta-like noncanonical notch ligand 2 regulates the proliferation and differentiation of sheep myoblasts through the Wnt/-catenin signaling pathway. J CELL PHYSIOL. 2024 Jul;:e31385Read more>>
VALIDATION IMAGES