bs-3550R
[Primary Antibody]
Myogenin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Myogenin
Immunogen Range: 80-125/224
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4656
Swiss Prot: P15173
Source: KLH conjugated synthetic peptide derived from human MYOG/Myogenin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 25
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Predicted Cross Reactive Species: Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Li S et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019 Apr 25.Read more>>
- Li S et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019 Apr 25.Read more>>
- Wang Y et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019 Nov 7;10(11):852.Read more>>
- Weng S et al. Improvement of muscular atrophy by AAVCSaCas9-mediated myostatin gene editing in aged mice. Cancer Gene Ther. 2020 Dec;27(12):960-975.Read more>>
- Zhao Wang. et al. Fibronectin type III domain-containing 4 promotes the migration and differentiation of bovine skeletal muscle-derived satellite cells via focal adhesion kinase. Cell Adhes Migr. 2020;14(1):153-164Read more>>
- Jie Li. et al. MicroRNA-127-3p regulates myoblast proliferation by targeting Sept7. Biotechnol Lett. 2020 Sep;42(9):1633-1644Read more>>
- Qiang Gao. et al. Myogenin suppresses apoptosis induced by angiotensin II in human induced pluripotent stem cell-derived cardiomyocytes. Biochem Bioph Res Co. 2021 May;552:84Read more>>
- Genghua Chen. et al. Transcriptome profile analysis reveals KLHL30 as an essential regulator for myoblast differentiation. Biochem Bioph Res Co. 2021 Jun;559:84Read more>>
- Kuan-Xiang Sun. et al. Deletion of phosphatidylserine flippase -subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration. Zool Res. 2021 Sep 18; 42(5): 650C659Read more>>
- Zhi Hu. et al. MUSTN1 is an indispensable factor in the proliferation, differentiation and apoptosis of skeletal muscle satellite cells in chicken. Exp Cell Res. 2021 Oct;407:112833Read more>>
- Yilei Li. et al. Vitellogenin 2 promotes muscle development and stimulates the browning of white fat. Aging-Us. 2021 Oct 15; 13(19): 22985C23003Read more>>
- Jing Zhang. et al. LncRNA SMARCD3-OT1 Promotes Muscle Hypertrophy and Fast-Twitch Fiber Transformation via Enhancing SMARCD3X4 Expression. INT J MOL SCI. 2022 Jan;23(9):451Read more>>
- Sheng-Zhong Han. et al. miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation. J AGR FOOD CHEM. 2022;70(33):1012110133Read more>>
- Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858Read more>>
- Pengkang Song. et al. Vitamin A injection at birth improves muscle growth in lambs. ANIM NUTR. 2023 JuRead more>>
- Genghua Chen. et al. Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation. CELL PROLIFERAT. 2023 Sep;:e13545Read more>>
- Yue Wu. et al. The biological characteristics of chicken embryo mesenchymal stem cells isolated from chorioallantoic membrane. GENESIS. 2024 Apr;62(2):e23592Read more>>
- Changbin Zhao. et al. IGF2 promotes the differentiation of chicken embryonic myoblast by regulating mitochondrial remodeling. J CELL PHYSIOL. 2024 JuRead more>>
- Junfang Zhang. et al. GSK3 regulation Wnt/-catenin signaling affects adipogenesis in bovine skeletal muscle fibro/adipogenic progenitors. INT J BIOL MACROMOL. 2024 Aug;275:133639Read more>>
- Lixia Sun. et al. Delta-like noncanonical notch ligand 2 regulates the proliferation and differentiation of sheep myoblasts through the Wnt/-catenin signaling pathway. J CELL PHYSIOL. 2024 Jul;:e31385Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti MYOG/MYOGENIN Polyclonal Antibody,Unconjugated (bs-3550R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: rat heart lysates Lane 2: mouse skeletal muscle lysates probed with Anti MYOG/MYOGENIN Polyclonal Antibody, Unconjugated (bs-3550R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 25kD. Observed band size: 25kD.
Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti MYOG/Myogenin Polyclonal Antibody, Unconjugated (bs-3550R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 25kD. Observed band size: 25kD,50kD.