bs-3543R-PE [Conjugated Primary Antibody]
NFKB p65(Ser276) Polyclonal Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFKB p65 Ser276

Modification Site: Ser276

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5970

Swiss Prot: Q04206

Source: KLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of Ser276

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Parker, Matthew, et al. "C11orf95-RELA fusions drive oncogenic NF-kB signalling in ependymoma." Nature (2014).Read more>>
  • Zhang, Nan, et al. "Effect of vasopressin on hippocampal injury in a rodent model of asphyxial cardiopulmonary arrest." Experimental and therapeutic medicine 11.4 (2016): 1385-1392.Read more>>
  • Chen L et al. Forsythiaside prevents β-amyloid-induced hippocampal slice injury by upregulating 2-arachidonoylglycerol via cannabinoid receptor 1-dependent NF-κB pathway. Neurochem Int. 2019 May;125:57-66.Read more>>
  • Gao R et al. Quasi-ultrafine particles promote cell metastasis via HMGB1-mediated cancer cell adhesion. Environ Pollut. 2019 Oct 23:113390. Read more>>
  • Fanyun Konget al. Hepatitis B virus core protein promotes the expression of neuraminidase 1 to facilitate hepatocarcinogenesis. Lab Invest. 2020 Dec;100(12):1602-1617.Read more>>
  • Xiaoli Gao. et al. MicroRNA-21-5p targets PDCD4 to modulate apoptosis and inflammatory response to Clostridium perfringens beta2 toxin infection in IPEC-J2 cells. Dev Comp Immunol. 2021 Jan;114:103849Read more>>
  • Sun J. et al. Plasma Exosomes Transfer miR-885-3p Targeting the AKT/NF_B Signaling Pathway to Improve the Sensitivity of Intravenous Glucocorticoid Therapy Against Graves Ophthalmopathy.. Front Immunol. 2022 Feb;13:819680-81968Read more>>
  • Xiao Wang. et al. Kallistatin attenuates inflammatory response in rheumatoid arthritis via the NF-B signaling pathway. EUR J PHARMACOL. 2023 Jan;:17553Read more>>
  • Zhen Xu. et al. Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Zhen Xu. et al. The aqueous extracts of Ageratum conyzoides inhibit inflammation by suppressing NLRP3 inflammasome activation. J ETHNOPHARMACOL. 2023 Jun;309:116353Read more>>
  • Kuangyang Yang. et al. Identification of Andrographolide as a novel FABP4 inhibitor for osteoarthritis treatment. PHYTOMEDICINE. 2023 Sep;118:154939Read more>>
  • Kaihui Xie. et al. Overexpression of SIRT1 alleviates oxidative damage and barrier dysfunction in CPB2 toxin-infected IPEC-J2 cells. MICROB PATHOGENESIS. 2023 Jun;:106181Read more>>
  • Li Wei. et al. The CXCL12-CXCR4-NLRP3 axis promotes Schwann cell pyroptosis and sciatic nerve demyelination in rats. CLIN EXP IMMUNOL. 2023 JulRead more>>
  • Zhang Tianyu. et al. Research on the Metabolic Regulation Mechanism of Yangyin Qingfei Decoction Plus in Severe Pneumonia Caused by Mycoplasma Pneumoniae in Mice. FRONT PHARMACOL. 2024 Mar;15Read more>>
  • Feng Gao. et al. Goat milk exosomal microRNAs alleviate LPS-induced intestinal inflammation in mice. INT J BIOL MACROMOL. 2024 May;268:131698Read more>>
VALIDATION IMAGES