Host: Rabbit
Target Protein: PIM1 + PIM3
Immunogen Range: 251-350/404
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5292
Source: KLH conjugated synthetic peptide derived from human PIM1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 45
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Sheep
Pig
Chicken
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Yumeng Cao. et al. PIM1 inhibition attenuated endotoxin-induced acute lung injury through modulating ELK3/ICAM1 axis on pulmonary microvascular endothelial cells. Inflamm Res. 2021 Jan;70(1):89-98Read more>>
Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti PIM1 Polyclonal Antibody, Unconjugated (bs-3540R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 45kD. Observed band size: 45kD.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with PIM1 Polyclonal Antibody, Unconjugated (bs-3540R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.