bs-3501R [Primary Antibody]
Synapsin 1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Synapsin 1

Specificity: This antibody will detect both isoforms of Synapsin 1.

Immunogen Range: 201-300/705


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6853

Swiss Prot: P17600

Source: KLH conjugated synthetic peptide derived from human Synapsin I

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 85


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Luo, Li, et al. "Niche astrocytes promote the survival, proliferation and neuronal differentiation of co‑transplanted neural stem cells following ischemic stroke in rats." Experimental and Therapeutic Medicine.Read more>>
  • Lin XY et al. Chronic exercise buffers the cognitive dysfunction and decreases the susceptibility to seizures in PTZ-treated rats. Epilepsy Behav. 2019 Aug 1;98(Pt A):173-187.Read more>>
  • Dan Wu et al. Impairment of learning and memory induced by perinatal exposure to BPA is associated with ER-mediated alterations of synaptic plasticity and PKC/ERK/CREB signaling pathway in offspring rats. Brain Res Bull. 2020 Aug;161:43-54.Read more>>
  • Hui Zhen. et al. The Wnt/Ca2+ signaling pathway is essential for the regeneration of GABAergic neurons in planarian Dugesia japonica. Faseb J. 2020 Dec;34(12):16567-1658Read more>>
  • Dan Wu. et al. Protective effect of alpha-lipoic acid on bisphenol A-induced learning and memory impairment in developing mice: nNOS and keap1/Nrf2 pathway. Food Chem Toxicol. 2021 Aug;154:112307Read more>>
  • Jian Ren. et al. Hydrophilic hindering and hydrophobic growing: a vesicle glycometabolism multi-drug combination therapeutic against Alzheimer's disease. Biomater Sci-Uk. 2021 AugRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat hippocampus labeled with Anti-SYN1/Synapsin 1 Polyclonal Antibody, Unconjugated (bs-3501R) at 1:300 followed by conjugation to the secondary antibody and DAB staining


Lane 1: HL60 cell lysates probed with Synapsin 1 Polyclonal Antibody, Unconjugated (bs-3501R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Synapsin 1 ) Polyclonal Antibody, Unconjugated (bs-3501R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.