bs-3498R [Primary Antibody]
p70 S6 Kinase Beta 2 (Tyr389) Antibody
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: p70 S6 Kinase Beta 2 Tyr389

Modification Site: Tyr389

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic phosphopeptide derived from human p70 S6 Kinase Beta 2 around the phosphorylation site of Tyr389

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 53


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hiyama et al. Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation. (2015) J.Vet.Med.Sc. 77:305-11Read more>>
VALIDATION IMAGES

Mouse bone lysates probed with p70 S6 Kinase Beta 2 (Tyr389) Polyclonal Antibody, Unconjugated (bs-3498R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.